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Iron and zinc are important minerals in humans in sub Saharan Africa, whose deficiency is known as "hidden hunger" due to the lack of recognised symptoms in the early stages. Although iron deficiency is the most prevalent, zinc is also involved in inhibition of replication of viruses, including the corona virus (COVID-19). In North Kivu and South Kivu provinces where more than 50% of common bean is produced and consumed in Democratic Republic of Congo, 36% and 47% of preschool children are anemic due to iron deficiency. This paradox is mainly due to insufficiency of iron-rich foods. The aim of this study is to characterise 59 iron and zinc biofortified varieties together with six local varieties of common bean for a potential selection programme in Butembo town in the Democratic Republic of Congo. We focused on 15 qualitative and five quantitative parameters. The qualitative parameters were helpful to distinguish the different morphotypes and for cluster analysis. In addition to the descriptive statistics, the quantitative data were used for Pearson correlation and for principal component analysis, PCA. Qualitative parameters enabled grouping of the study genotypes into 14 morphotypes according to the aspect and colour of the seed coat, the colour around the hilum and the size of seeds. Clustering grouped the 65 genotypes into 12 clusters with the most similar genotypes grouped in the same cluster. Quantitative parameters showed that the study genotypes were dissimilar (P=0.00). A positive correlation was obtained between the days to flowering and the days to maturity (P<0.05) and between the number of pods per plant and the days to flowering. A strong correlation was found between the number of pods per plant and seeds per pod (P<0.01). In contrast, a negative correlation was observed between the 100 seed weight and the number of seeds per pod. The PCA represented on two perpendicular axes showed 64.1% of the total variance of which the 42.3% is explained by the first axis and 21.8% by the second axis. Overall, the study genotypes are morphologically and quantitatively different and thus can be used in a selection programme.
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This paper presents the evaluation results of the yield target setting precision of the revised MOET App (v.2.0), following the inclusion of the correction factors generated from rice biomass correlations between MOET and nutrient omission plot setups obtained from 2017 to 2018. The project started with trainings on MOET kit and MOET App use for the seed production personnel across PhilRice stations in Nueva Ecija, Negros, Bukidnon and Agusan in 2018 DS. Included in the trainings were the establishment of MOET kit tests and generation of variety- and site-specific recommendations via the MOET App for several nationally or regionally recommended varieties (NSIC Rc 122, 160, 216, 218, 222, 238, 286, 300, 358, 402, 436, 440, 442, 480, PSB Rc18 and PSB Rc82) that each PhilRice station intended to produce in the succeeding 4 cropping periods from 2019 to 2020. Relative yield advantages and economic benefits from using the MOET App fertilizer recommendations over PhilRice' current fertilizer management in seed production per station were monitored every cropping while the precision evaluation of yield target setting was done after the last cropping of 2020 WS. In 2019, relative yield advantages averaged 0.43t ha-1 in DS and 0.25t ha-1 in WS. In 2020 DS, an average relative yield advantage of 0.63t ha-1 was obtained across stations and 0.93t ha-1 in 2020 WS in Nueva Ecija only due to travel restrictions brought about by the COVID-19 pandemic. Economic benefits of using the MOET App showed an average of 0.50t ha-1 and 0.65t ha-1 yield increase over the seed production units' fertilizer management in DS and WS, respectively. While savings in fertilizer cost were better realized during the WS at an average of Php 4,126.34 ha-1 season-1 across stations. Results of the precision evaluation of the yield target showed marked improvements with a 95.24% probability of achieving 17% higher grain yields than the target set by MOET App v.2.0. However, the overall normalized Root Mean Square Error (nRMSE) of 38.14% exceeded the range for a fairly acceptable fit with the model due to large gaps between target and actual yields obtained from DS field trials.
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The main food consumption of farming families is an important concern during the pandemic. Farmers in West Nusa Tenggara (WNT) survive with the availability of rice to support household health and food security during the pandemic. In addition to its nutritional content, rice also contains phenolic compounds and has antioxidant activity. This study aims are to measure the frequency of rice consumption, food variety, varieties of rice consumed daily during the pandemic in WNT, as well as the antioxidant properties of several rice varieties grown in WNT. This study was conducted from September 2020 to June 2021 in East Lombok, Sumbawa and Bima Regencies, WNT Province, Indonesia using a survey approach. The data collection techniques were observation, Focus Group Discussion, recording, interviews with 74 respondents with a semi-structured questionnaire and study literature related to the nutritional content and bioactive compound of the rice. Quantitative data was tabulated to determine the frequency and average then descriptively analyzed, while qualitative data was thematically analysed. The most consumed rice varieties are seen from the production data of seed breeders in the WNT region, the phenolic content was determined using Folin-Ciocalteu, antioxidant activity was examined using DPPH assay. The result showed that the highest portion of food consumed by farmer households in WNT is rice. The most variety of rice produced by breeders is the Inpari 32 variety. There are about 97% of farmers consume rice for 3 times a day, while 3% of the remaining consume 2 times a day of rice. Food variety includes 7.9% vegetable-rice and 92.1% rice-vegetables-meat protein. The results of the analysis of antioxidant properties and phenolic content in rice is depending on the variety. The antioxidant properties of the Jeliteng, Baroma, Pamelen, Nutrizinc and Inpari 32 rice varieties were 44.85+or-0.51%, 9.87+or-1.55%, 9.96+or-1.22%, 9.75+or-1.09%, and 14.305+or-1.24%, respectively, while the phenolic contents were 9.76+or-0.09, 4.23+or-0.03, 4.48+or-0.02, 4.64+or- 0.07, 4.58+or-0.42 mg GAE/g dryrice extract, respectively. The results of this study indicate that rice has been used as one of antioxidants source for farming families during the pandemic.
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Andhra Pradesh is one of the major tomato producing states in the country. The presents tudy was conducted to identify the adoption pattern of 448 variety of tomato in Ananthapuramu district of Andhra Pradesh which was released by IIHR, Bangalore. The study also includes identification of the major factors for adoption and find out the major attributes of innovation that led to the adoption of 448 variety. An ex-post facto research design was adopted for the study. The study included 40 respondents chosen by purposive sampling method. Data was collected with the help of well-structured interview schedule through telephonic interview due to COVID-19 pandemic. The statistical tools used for analysis are mean, standard deviation, cumulative frequency and percentage analysis. Profile characters of the respondents viz., age, educational status, occupation, farm size, farming experience, annual income, frequency of exposure to agricultural messages, scientific orientation and cropping pattern were studied. Based on the mean and SD values the respondents were classified into adopter categories. The results of the adopter categorization showed that two-fifths (40.00%) of respondents belonged to early majority and only 5.00 percent were innovators. Among the factors affecting adoption of 448 variety of tomato, majority (80.00%) adopted due to their own interest and with the influence of friends and neighbours. Compatibility (95.00%) and observability (82.50%) of the variety played an important role among the attributes of innovation for the adoption of 448 variety of tomato among the respondents.
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In the Republic of Kazakhstan melons and gourds are produced mainly in the traditional way, that is, by using chemical fertilizers and pesticides. The production of environmentally friendly melons is at the initial stage of its development. There is no organic melon growing at all. Considering the huge export potential of melons among other crops cultivated in the country (the total supply of melons and melons on the domestic market is 472%), the great economic and social significance of the transition of the melon industry to organic production is growing. In the future, Kazakhstan may be in the center of attention of the world community as a manufacturer and supplier of environmentally friendly (organic) melons, and environmentally friendly, natural melons may well become a brand of our sovereign state. To do this, it is necessary to make fundamental changes in the melon industry of the country. In general, the transition of agricultural production to an organic direction will contribute to the consumption of the safest and most useful products by the local population. Here, one should also take into account the decrease in the immunity of the human body due to various diseases, including those associated with the coronavirus pandemic. It should also be taken into account that only a limited number of pesticides used against harmful objects in agriculture act as intended, and most of the toxic substances spread into the environment. The use of disinfectants without scientific support has a negative impact on flora and fauna, being deposited in the soil and irrigation system of fields for a long time, thereby causing enormous damage to the environment. Given the above facts, our scientific research was aimed at the selection of pesticides and biological preparations with high biological and economic efficiency, low rates and frequency of treatments, as well as varieties with high resistance to fungal diseases of melons (watermelon, melon). Research work was carried out on the experimental plots of the Regional Branch "Kainar" of the LLP "Kazakh Research Institute of Horticulture" in the period 2020-2021. The studies used classical methods generally accepted in melon growing. The results obtained are new and relevant, and in the future, will contribute to the transition from traditional melon growing to biological.
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Various kinds of field crops growing on two commercial farms in the Whitehorse area of the southern Yukon Territory were surveyed for diseases in summer 2020 by staff of the Agriculture Branch of the Government of Yukon. They included barley, wheat, canola, beets, broccoli, cabbage, carrots, potatoes and turnips. Fields were visited one or more times during July and August. The incidence and severity of diseases were visually assessed on a crop-by-crop basis and samples were collected for laboratory analysis of the pathogens present, if any. Both infectious and non-infectious diseases were present on most crops. The infectious diseases were caused by various species of plant pathogenic bacteria and fungi that were common on these crops growing in other areas of Canada. INTRODUCTION AND METHODS: The 2020 field crop disease survey is believed to be the first organized study of its kind on agricultural crops in the Territory. In his book, "An Annotated Index of Plant Diseases in Canada . . . ", I.L. Conners lists over 300 records of plant diseases on trees, shrubs, herbs and grasses in the Yukon that were published by individuals who were surveying forests and native vegetation mainly for federal government departments, universities and other agencies (Conners 1967). The objectives of the 2020 survey were: (1) to determine the kinds and levels of diseases on selected Yukon crops, (2) to identify the major pathogen species attacking Yukon crops, and (3) to use the results to plan future surveillance activities aimed at helping producers to improve their current disease management programs. All of the fields included in the 2020 survey were situated on two commercial farms, which were designated as Farm #1 and #2, in the Whitehorse area in the southern Yukon (Fig. 1). The crops surveyed included cereals (barley and wheat), oilseeds (canola) and vegetables (beets, broccoli, cabbage, carrots, potatoes and turnips). Fields were visited one or more times in the mid- to late growing season (July/August) at a time when damage from diseases was most noticeable. Symptoms were visually assessed on a crop-by-crop basis by determining their incidence and severity. Incidence was represented by the percentage of plants, leaves, heads, kernels, etc., damaged in the target crop, while severity was estimated to be the proportion of the leaf, fruit, head, root/canopy area, etc., affected by a specific disease as follows: Proportion of the canopy affected based on a 0-4 rating scale, where: 0 = no disease symptoms, 1 = 1-10% of the crop canopy showing symptoms;2 = 11-25% showing symptoms, 3 = 26- 50% showing symptoms, and 4 = > 50% showing symptoms. Photographs of affected plants were taken and sent to plant pathologists across Western Canada for their opinions on causation. Where possible, representative samples of plants with disease symptoms were packaged and sent to the Alberta Plant Health Lab (APHL) in Edmonton, AB for diagnostic analyses. Background information, such as the general cultural practices and cropping history, was obtained from the producers wherever possible. GPS coordinates were obtained for each field to enable future mapping Cereals: Individual fields of barley (11 ha) and wheat (30 ha) located at Farm #1 were surveyed. The barley was a two-row forage cultivar 'CDC Maverick', while the wheat was an unspecified cultivar of Canada Prairie Spring (CPS) Wheat. Plant samples were taken along a W-shaped transect for a total of five sampling points for the barley field (< 20 ha) and ten sampling points for the wheat field (> 20 ha). The first visit, which occurred on July 30, involved visual inspection and destructive sampling wherein plants were collected and removed from the field for a detailed disease assessment at a lab space in Whitehorse. There, the roots were rinsed off and the plants were examined for disease symptoms. The second visit to these fields, which occurred on August 27, only involved visual examination of the standing crop. Oilseeds: A single 40 ha field of Polish canola (cv. 'Synergy') was examined o
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Seeking to fine-tune nitrogen (N) application, increase economic returns, and decrease environmental N loss, some Arkansas rice (Oryza sativa L.) producers are turning away from blanket N recommendations based on soil texture and cultivar and using the Nitrogen Soil Test for Rice (N-STaR) to determine their field-specific N rates. In 2010, Roberts et al. correlated years of direct steam distillation (DSD) results obtained from 0- to 18-in. soil samples to plot-scale N response trials across the state to develop a field-specific, soil-based N test for Arkansas rice. After extensive small-plot and field-scale validation, N-STaR is available to Arkansas farmers for both silt loam and clay soils. Samples submitted to the N-STaR Soil Testing Lab in 2021 were summarized by county and soil texture, totaled 21 fields across 9 Arkansas counties, and were from 6 clay and 15 silt loam fields. Depressed sample submissions were again observed likely due to another wet spring and lingering effects of the COVID-19 pandemic. The N-STaR N-rate recommendations for samples were compared to the producer's estimated N rate, the 2021 Recommended Nitrogen Rates and Distribution for Rice Cultivars in Arkansas, and the standard Arkansas N-rate recommendation of 150 lb N/ac for silt loam soils and 180 lb N/ac for clay soils. Each comparison was divided into 3 categories based on a decrease in recommendation, no change in recommended N rate, or an increase in the N rate recommendation. In all 3 comparisons, county, but not soil texture, was a significant factor (P < 0.04) in observed decreases in N recommendation strategies demonstrating variations in the soil's ability to supply N across the state. Further stressing the potential N cost savings opportunities, reductions greater than 30 lb N/ac were recommended by N-STaR in 71%, 50%, and 74% of fields in the standard, estimated, and cultivar comparisons, respectively.
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Indonesia is a country with a very high diversity of bananas (Musa spp.). The diversity of bananas can be found in Indonesia ranging from wild to commercial ones. Banana research activities at Indonesian Tropical Fruit Research Institute (ITFRI) started with the Musa germplasm collection, comprising local cultivars from several regions in Indonesia in 1987. In 1995, ITFRI introduced several accessions of bananas and plantains from International Network for the Improvement of Banana and Plantain (INIBAP), and at about the same time Musa exploration activities intensively took place to collect both local cultivars and wild species in several main islands of Indonesia. In line with the collection of Musa genetic materials, cultivar development activities were also carried out, including the selection and evaluation of superior characters of local and introduced cultivars, and breeding programs to produce superior quality and disease-tolerant bananas. Some of the superior cultivars resulting from selection and hybridization that have been released were, Ketan-01, Kepok Tanjung, Raja Kinalun, INA-03, and Sang Mulyo. With the outbreak of the current COVID-19 pandemic, public awareness of fruit consumption, including bananas, has also increased. The availability of superior disease-tolerant and high-yielding cultivars such as Kepok Tanjung and Sang Mulyo will greatly assist the availability of domestic bananas.
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This study was conducted to investigate the influence of pumpkin cultivar as a fermented semi-product on the selected quality parameters of functional non-dairy frozen desserts, which were prepared using the potentially probiotic strain cultures L. rhamnosus Lock 0900 and L. casei O14. Microbiological status, pH, sugar and carotenoids content, antioxidant activity, color and sensory quality have been checked. Regardless of the pumpkin cultivar used, the lactic acid fermentation of pulps with selected bacterial strains decreased reducing sugar and total sugars content. Improvement in the antioxidant activity was also observed. The impact of the individual sensory attributes on the overall quality was determined. The overall quality of the investigated pumpkin sorbets was positively driven by the sweet taste, pumpkin flavor, smoothness of texture, and negatively driven by acidic flavor, pungent taste, and bitter taste, verified by PCA method. All of the final products achieved high survival of probiotics (higher than 8.4 log CFU/g) and revealed a good sensory quality (overall quality higher than 8 c.u). The treatments with the cultivar “Melon Yellow” of the Cucurbita maxima species, were characterized by significantly higher (p < 0.05) carotenoids content, total sugars and reducing sugars content and antioxidant activity, measured by two methods. It can be concluded that the pumpkin cultivar and strain culture used for the fermentation affect the count of potentially probiotic bacteria in the final products, the composition of bioactive compounds, antioxidant activity and sensory quality of the functional pumpkin frozen desserts.
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Originally, the 17th Symposium on Insect-Plant Relationships (SIP-17) was scheduled to take place in Leiden, The Netherlands, in July 2020. However, due to the COVID-19 pandemic, the symposium was postponed to July 2021 and held in an exclusively online format. This exceptional edition has resulted in four strong contributions to the journal. It is with great pleasure that we now present a themed issue including the proceedings of SIP-17, supplemented with eight regular articles within the subject of insect-plant relationships.
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Asian soybean rust (ASR) caused by Phakopsora pachyrhizi is a major threat to soybean production in Bangladesh. Understanding the yearly changes and the current status of pathogenic structures is essential for developing appropriate breeding strategies for obtaining ASR‐resistant soybean lines. Thirty‐four P. pachyrhizi samples were collected from ASR hotspot areas (Chandpur, Lakshmipur, Noakhali, Barisal and Bhola districts) of Bangladesh in 2018 and 2019 and evaluated for pathogenicity on 12 soybean differential lines. The tested samples showed similar and dissimilar pathogenicity patterns on the differentials, yielding 21 distinct pathotypes. The cluster analysis, principal coordinate analysis and principal component analysis of the disease phenotypes of 47 samples collected in 2016, 2018 and 2019 indicated a higher pathogenic diversity and virulence variation in the P. pachyrhizi samples of 2018 and 2019 compared to that of 2016. The pathogenicity profiles of the Bangladeshi P. pachyrhizi samples appeared distinct from those of Argentinian and Brazilian samples, but showed slight similarities with Japanese, Mexican and Paraguayan samples. Furthermore, none of the resistance genes for P. pachyrhizi (Rpp genes) was solely effective against all the tested samples from 2018 and 2019, while samples (BdRP‐48, BdRP‐56 and BdRP‐58) virulent to all Rpp1–Rpp6 genes were detected. The Rpp‐pyramided line No6–12–1, carrying Rpp2, Rpp4 and Rpp5, was capable of conferring robust resistance to these virulent samples. Altogether, these results indicate an increase in the virulence of the current ASR pathogen in Bangladesh, which can be resolved by pyramiding different resistance genes in soybean cultivars. [ FROM AUTHOR] Copyright of Plant Pathology is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full . (Copyright applies to all s.)
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The normal management of plant germplasm conservation laboratories involves carrying out numerous and diverse activities, which were affected by the coronavirus pandemic (COVID-19). The objective of this publication was to review the evolution of the cassava in vitro germplasm bank at the FCA-UNNE and IBONE (CONICET-UNNE) and to tell about usual management practices and the procedures to preserve living plant material and the personnel's life involved in pre-pandemic and COVID-19 pandemic times. Teachers, researchers, undergraduate and graduate students carried out, for almost 40 years, the in vitro conservation of 56 cassava cultivars from different countries. Before March 2020, the bank management consisted mainly in scientific-technological activities for the conservation of the material and the search for parameters to establish an order of subcultures. Having decreed Social, Preventive and Compulsory Isolation in Argentina, conservation activities continued applying the usual practices by following political-institutional sanitary measures. To face the new sanitary scenarios, methodologies must be adjusted so that they are effective at maintaining viability of the plant material and at prolonging conservation time.
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Numerous compact tomato cultivars are available for home gardening. However, evaluations under different environmental conditions are limited. The aim of this study was to characterize the growth and productivity of 20 compact tomato cultivars grown indoors under environmental conditions that resembled a residential space (11 mol·m−2·d−1 of white light, constant 22 °C, and moderate relative humidity) or in a greenhouse with sunlight only. Plants in the greenhouse were generally larger and yielded more fruit than those grown indoors, likely due to the various differences in environmental conditions and corresponding effects of water and nutrient availability. Considering growth and yield variables, all cultivars evaluated in this study are recommended for outdoor gardening. However, ‘Little Bing’, ‘Sweet SturdyTM F1—Grace’, ‘Sweet SturdyTM F1—Jimmy’, ‘Sweet SturdyTM F1—Jo’, and ‘Tarzan F1′ are likely too large to be grown in most space-limited indoor environments. Furthermore, ‘Little Bing’, ‘Rosy Finch’, ‘Sweet ‘n’ Neat Yellow’, and ‘Yellow Canary’ were affected by intumescence when grown indoors, which could negatively affect gardening experiences until recommendations to mitigate this disorder become available. Results from this study provide baseline information for the use of compact tomato cultivars for container gardening indoors and under sunlight.
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Around 2 billion people are suffering from chronic malnutrition or “hidden hunger”, which is the result of many diseases and disorders, including cognitive degeneration, stunting growth, and mortality. Thus, biofortification of staple food crops enriched with micronutrients is a more sustainable option for providing nutritional supplements and managing malnutrition in a society. Since 2001, when the concept of biofortification came to light, different research activities have been carried out, like the development of target populations, breeding or genetic engineering, and the release of biofortified cultivars, in addition to conducting nutritional efficacy trials and delivery plan development. Although, being a cost-effective intervention, it still faces many challenges, like easy accessibility of biofortified cultivars, stakeholders’ acceptance, and the availability of biofortified germplasm in the public domain, which varies from region to region. Hence, this review is focused on the recent potential, efforts made to crop biofortification, impacts analysis on human health, cost-effectiveness, and future perspectives to further strengthen biofortification programs. Through regular interventions of sustainable techniques and methodologies, biofortification holds huge potential to solve the malnutrition problem through regular interventions of nutrient-enriched staple food options for billions of people globally.
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The coffee leaf miner (Leucoptera coffeella) is a primary pest for coffee plants. The attack of this pest reduces the photosynthetic area of the leaves due to necrosis, causing premature leaf falling, decreasing the yield and the lifespan of the plant. Therefore, this study aims to analyze vegetation indices (VI) from images of healthy coffee leaves and those infested by coffee leaf miner, obtained using a multispectral camera, mainly to differentiate and detect infested areas. The study was conducted in two distinct locations: At a farm, where the camera was coupled to a remotely piloted aircraft (RPA) flying at a 3 m altitude from the soil surface;and the second location, in a greenhouse, where the images were obtained manually at a 0.5 m altitude from the support of the plant vessels, in which only healthy plants were located. For the image processing, arithmetic operations with the spectral bands were calculated using the “Raster Calculator” obtaining the indices NormNIR, Normalized Difference Vegetation Index (NDVI), Green-Red NDVI (GRNDVI), and Green NDVI (GNDVI), the values of which on average for healthy leaves were: 0.66;0.64;0.32, and 0.55 and for infested leaves: 0.53;0.41;0.06, and 0.37 respectively. The analysis concluded that healthy leaves presented higher values of VIs when compared to infested leaves. The index GRNDVI was the one that better differentiated infested leaves from the healthy ones.
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ETHNOPHARMACOLOGICAL RELEVANCE: Red sage (Lantana camara L.) (Verbenaceae) is a widely spread plant that was traditionally used in Brazil, India, Kenya, Thailand, Mexico, Nigeria, Australia and Southeast Asia for treating several ailments including rheumatism and leprosy. Despite its historical role in relieving respiratory diseases, limited studies progressed to the plant's probable inhibition to respiratory viruses especially after the striking spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections. AIM OF THE STUDY: This study aimed to investigate the inhibitory activity of different L. camara cultivars to SARS-CoV-2, that was not previously inspected, and clarify their mechanisms of action in the metabolomics viewpoint, and to determine the biomarkers that are related to such activity using UPLC-MS/MS coupled to in vitro-studies and chemometric analysis. MATERIALS AND METHODS: Chemical profiling of different cultivars was accomplished via UPLC-MS/MS. Principle component analysis (PCA) and orthogonal projection to latent structures (OPLS) models were built using SIMCA® (multivariate data analysis software). Cytotoxicity and COVID-19 inhibitory activity testing were done followed by TaqMan Real-time RT-PCR (Reverse transcription polymerase chain reaction) assay that aimed to study extracts' effects on RNA-dependent RNA polymerase (RdRp) and E-genes expression levels. Detected biomarkers from OPLS analysis were docked into potential targets pockets to investigate their possible interaction patterns using Schrodinger® suite. RESULTS: UPLC-MS/MS analysis of different cultivars yielded 47 metabolites, most of them are triterpenoids and flavonoids. PCA plots revealed that inter-cultivar factor has no pronounced effect on the chemical profiles of extracts except for L. camara, cultivar Drap d'or flowers and leaves extracts as well as for L. camara cv Chelsea gem leaves extract. Among the tested extracts, flowers and leaves extracts of L. camara cv Chelsea gem, flowers extracts of L. camara cv Spreading sunset and L. camara cv Drap d'or showed the highest selectivity indices scoring 12.3, 10.1, 8.6 and 7.8, respectively, indicating their relative high safety and efficacy. Leaves and flowers extracts of L. camara cv Chelsea gem, flowers extracts of L. camara cv Spreading sunset and L. camara cv Drap d'or were the most promising inhibitors to viral plaques exhibiting IC50 values of 3.18, 3.67, 4.18 and 5.01 µg/mL, respectively. This was incremented by OPLS analysis that related their promising COVID-19 inhibitory activities to the presence of twelve biomarkers. Inhibiting the expression of RdRp gene is the major mechanism behind the antiviral activity of most extracts at almost all concentration levels. Molecular docking of the active biomarkers against RdRp revealed that isoverbascoside, luteolin-7,4'-O-diglucoside, camarolic acid and lantoic acid exhibited higher docking scores of -11.378, -10.64, -6.72 and -6.07 kcal/mol, respectively, when compared to remdesivir (-5.75 kcal/mol), thus these four compounds can serve as promising anti-COVID-19 candidates. CONCLUSION: Flowers and leaves extracts of four L. camara cultivars were recognized as rich sources of phytoconstituents possessing anti-COVID-19 activity. Combination of UPLC-MS/MS and chemometrics is a promising approach to detect chemical composition differences among the cultivars and correlate them to COVID-19 inhibitory activities allowing to pinpoint possible biomarkers. Further in-vitro and in-vivo studies are required to verify their activity.
Subject(s)
COVID-19 Drug Treatment , Lantana , Biomarkers/analysis , Chemometrics , Chromatography, High Pressure Liquid , Chromatography, Liquid , Lantana/chemistry , Molecular Docking Simulation , Plant Extracts/analysis , Plant Extracts/pharmacology , Plant Leaves/chemistry , SARS-CoV-2 , Tandem Mass SpectrometryABSTRACT
Phosphorus (P) is one of the essential nutrients for alfalfa (Medicago sativa L.) growth, but P deficiency in soil is a common phenomenon. Applying an appropriate amount of P fertilizer for alfalfa in the establishment year improves winter survival, helps achieve sustainable high yields, and promotes the best economic returns. The objective of this study was to determine the effect of different P fertilization rates (0, 22, 44, and 66 kg P ha−1) on winter survival, forage yield, and root traits of two late-summer seeded alfalfa cultivars (dormant and semi-dormant) in the establishment and first production year. Our results showed that applying an appropriate amount of P fertilizer can increase forage yield and enhance the persistence of late-summer seeded alfalfa by promoting the development of crown and root traits and improving winter survival, especially for semi-dormant cultivars. Late-summer seeding and application of appropriate P fertilizer rates are effective agronomic methods for alfalfa in cold and arid winter regions, and can be used as strategies to improve fertility and stabilize soil from wind erosion for sustainable agriculture. To obtain high rate of survival in winter, the optimal P fertilization rates for the dormant and semi-dormant alfalfa cultivars in this study were 31.4 and 41.3 kg P ha−1, whereas the optimum P application rates for high forage yields were 31.3 and 31.5 kg P ha−1, respectively.
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The global COVID pandemic has impacted the world in ways and at a scale that few could have predicted, with many industries severely disrupted. Despite this, crops were sown and harvested, food was produced and agriculture continued to function, albeit it with many logistical challenges. Plant health lies at the heart of preventing crop losses through a combination of varietal resistance and agronomic practices. In the case of foliar plant diseases in wheat, varietal resistance plays a key role, but the use of synthetic fungicides is essential to minimize crop losses. European arable production faces a dilemma: how to contribute and maintain global food supplies but at the same time decrease emissions of greenhouse gases (GHGs), reduce inputs potentially harmful to biodiversity, society and the environment while ensuring no more land is brought into production. Throughout history, major disruptions in society have led to big steps in agricultural innovation. Presently, the major disruptive forces in Europe are not just a result of the COVID pandemic but the increasingly urgent need to address climate change. Within the European Green Deal, the Farm-to-Fork strategy is in place to help achieve climate neutrality by 2050, aiming for a reduction of GHG emissions of 55% by 2030. To achieve this, there will need to be a major adjustment to how food is produced, a realignment in plant health strategies and accelerated innovation across the agricultural sector. This paper aims to evaluate how synthetic fungicides presently contribute to plant health (mainly wheat) and food production as well as the management of GHG emissions. In addition, it explores the future challenges and prospects for their positive contribution in achieving global food security alongside emerging innovative technologies.
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Given that an effective combined foliar application of iodine (I), selenium (Se), and zinc (Zn) would be farmer friendly, compared to a separate spray of each micronutrient, for the simultaneous biofortification of grain crops, we compared effectiveness of foliar-applied potassium iodate (KIO3, 0.05%), sodium selenate (Na2SeO4, 0.0024%), and zinc sulfate (ZnSO4∙7H2O, 0.5%), separately and in their combination (as cocktail) for the micronutrient biofortification of four Basmati cultivars of rice (Oryza sativa L.). Foliar-applied, each micronutrient or their cocktail did not affect rice grain yield, but grain yield varied significantly among rice cultivars. Irrespective of foliar treatments, the brown rice of cv. Super Basmati and cv. Kisan Basmati had substantially higher concentration of micronutrients than cv. Basmati-515 and cv. Chenab Basmati. With foliar-applied KIO3, alone or in cocktail, the I concentration in brown rice increased from 12 to 186 µg kg−1. The average I concentration in brown rice with foliar-applied KIO3 or cocktail was 126 μg kg−1 in cv. Basmati-515, 160 μg kg−1 in cv. Chenab Basmati, 153 μg kg−1 in cv. Kisan Basmati, and 306 μg kg−1 in cv. Super Basmati. Selenium concentration in brown rice increased from 54 to 760 µg kg−1, with foliar-applied Na2SeO4 individually and in cocktail, respectively. The inherent Zn concentration in rice cultivars ranged between 14 and 19 mg kg−1 and increased by 5–6 mg Zn per kg grains by foliar application of ZnSO4∙7H2O and cocktail. The results also showed the existence of genotypic variation in response to foliar spray of micronutrients and demonstrated that a foliar-applied cocktail of I, Se, and Zn could be an effective strategy for the simultaneous biofortification of rice grains with these micronutrients to address the hidden hunger problem in human populations.
ABSTRACT
Rice is the world's largest food crop, and its production needs to be doubled by 2050 to cope with population growth and associated demand. In addition to the value of improving yields, quality is also important for breeders and consumers, but it pays less attention in arid regions. During two successive summer growing seasons, the experimental material focused on 34 genotypes developed from different crosses on Fn generation after fixation as well as six of the most recent commercial cultivars used for comparisons. The results showed that a high yield of grain followed by high milling and grain quality characteristics were observed among the 34 genotypes used in this analysis. Highly important and positive correlations between the percentage of hulling and the percentage of milling (0.424) and the yield ability could be accomplished by choosing the number of panicles per plant and the weight of the panicles. Selection criteria for good quality should be met by the percentage of head rice and many mineral elements, particularly zinc and iron. As a consequence, the genotypes M.J 5460S/SK105-1, M.J 5460S/GZ7768-1, M.J 5460S/G177-1, M.J 5460S/SK105-3 and M.J 5460S/SK106-4 had desirable high yield and quality characteristics and could be used as promising accessions to the rice breeding program in arid regions. In addition to commercial genotypes, improved Japonica rice genotypes could be produced in arid conditions for higher yield and quality, leading to an increase in total production, supporting food security and nutrition.